NRSP-8 Swine Genome Committee Annul Meeting Minutes

January 1, 2000 to December 31, 2000
Annual Meeting Date: January 14, 2001
Report Date: January 28, 2001

Participants:

State Agricultural Experiment Stations:

Illinois	J. Beever (Secretary, 2000)
Iowa		M. Rothschild, C. Tuggle
Kansas		D. Troyer
Michigan	C. Ernst (Chair, 2000)
Minnesota	M. Rutherford, L.Alexander, A. Paszek, L. Schook
Nebraska	D. Pomp (not present at meeting, written report submitted)
Nevada	A. Rink (new participating station)

U.S. Department of Agriculture:

USDA-BARC	J. Lunney
USDA-BARC	S. Fahrenkrug

Brief summary of minutes of annual meeting

The fifth annual Swine Workshop for the NRSP-8 swine species committee members and other attendees was held in conjunction with the Plant and Animal Genome IX conference in San Diego, CA on January 14, 2001. The meeting was held simultaneously with the annual meeting of the multistate research committee NC-210. Four invited speaker presentations were given, along with individual station reports and business meetings for NRSP-8 and NC-210. At the NRSP-8 business meeting, the current secretary, Jon Beever, assumed the role of chair for 2001 and Max Rothschild was elected as the new secretary. The committee voted to hold the 2001 annual meeting in conjunction with PAG X in San Diego, CA in January 2002. Minutes of the meeting and the species Coordinator's report are posted on the U.S. Pig Genome Coordination home page at http://www.genome.iastate.edu/pig.

Accomplishments and Impacts

Objective 1: Develop high resolution comparative genome maps aligned across species that link agricultural animal maps to those of the human and mouse genomes.

Several stations (IL, IA, MI, MN, NE) are continuing to map Type I comparative loci using the INRA somatic cell hybrid panel (SCHP) and the INRA-Minnesota porcine radiation hybrid (IMpRH) panel. The IA and MI stations are also identifying single-nucleotide polymorphisms (SNPs) at Type I loci for genetic linkage mapping. Numerous genes, selected based on their comparative location on the human map or for their physiological function, have been mapped in the past year. The IL and MN stations are emphasizing mapping of markers surrounding syntenic chromosomal breakpoints between the human and pig genomes. They are using the Comparative Mapping by Annotation and Sequence Similarity (COMPASS) approach to assimilate marker loci to improve the pig-human comparative map. Expressed sequence tag (EST) projects are underway at several stations (IL, IA, MI, MN, NE, NV, USDA-MARC). These projects are making thousands of new gene sequences available for comparative mapping. The USDA-MARC station is identifying SNPs in ESTs and placing them on the genetic linkage map. Other stations are using the SCHP and IMpRH panel for physical mapping of ESTs. The IL and NV stations have initiated projects to refine the fine scale pig physical map. The NV station is sequencing SLA II and the IL station is using BAC fingerprinting to construct a genome wide physical map. Competitive RT-PCR assays for critical cytokines (IFNg, IL-2, IL-3, IL-4, Il-5, Il-10, Il-12, Il-13. IL-15, IL-18) have been developed at USDA-BARC and are being used to determine gene expression changes in response to infectious diseases. The KS station demonstrated that bone marrow stromal cells could colonize pig fetal tissues and they also induced mesenchymal stem cells to a phenotype resembling neural stem cells and young neurons. These strategies may be useful for enhancing animal production efficiency in the future.

Objective 2: Increase the marker density of existing linkage maps used in QTL mapping and integrate them with physical maps of animal chromosomes.

The KS, MI and MN stations contributed new anonymous markers to the pig RH and genetic linkage maps. The KS station added a total of 15 microsatellite markers to the genetic maps of SSC8 and SSC13. The MI station added 18 markers identified using representational difference analysis (RDA) to the RH map and also placed nine of these on the genetic map. The MN station is involved in a large project to identify 1000 new microsatellite markers. To date they have over 300 candidate loci and sequence analysis and RH mapping of these markers is underway. The MN station is also fine mapping the region of a Quantitative Trait Locus (QTL) for number of copora lutia/ovulation rate on SSC8 and 10 new microsatellite markers have been mapped to this region. The development of 3 new resource populations for the identification of growth, meat quality and carcass merit QTL was reported by the IL, IA and MI stations. A Berkshire x Duroc F2 population is under development in IL and a Duroc x Pietrain F2 population is under development in MI. A Berkshire x Yorkshire F2 population at the IA station has been completed and 40 traits were measured on 525 animals. A primary genome scan using 125 microsatellite markers revealed over 100 putative QTL significant at the chromosome wide level and 19 of these were significant at the genome wide level.

Objective 3: Expand and enhance internationally shared species genome databases and provide other common resources that facilitate genome mapping.

Tissue specific EST libraries have been constructed at several stations (IL, IA, MI, MN, NE, NV, USDA-MARC). Tissues represented by these libraries include skeletal muscle, placenta, anterior pituitary, ovary, conceptus, fetus, hypothalmas, alveolar macrophages, peripheral blood cells, spleen, thymus, lymph node and bone marrow. Sequence information for these ESTs is being deposited into public databases and is available to pig geneticists and physiologists. Several stations (IA, MI, NV) are developing databases to catalogue information generated by their EST projects. In addition, the Pig Genome Coordinator oversaw significant updating of the Pig Genome Database (http://www.genome.iastate.edu) including the addition of new information on the maps and mapping tools.

Impacts:

Work done by the Swine Genome Committee of NRSP-8 has significantly enhanced the pig comparative map. The mapping of additional genes and ESTs facilitates the usefulness of the recently published human genome sequence because maps comparing the location of genes on human and mouse chromosomes with their location on pig chromosomes provide a rich source of candidate genes for QTL. A significant number of markers were added to the pig genetic linkage map. These markers included both Type I (gene) and Type II (anonymous) markers. These new markers will aid in the positional cloning of genes at QTL because having many markers available in regions harboring QTL considerably speeds the process of determining the exact position of the QTL and leads to its eventual identification. Once the genes at QTL are identified, it will be possible to improve the efficiency and reduce the costs of pork production by improving the speed and accuracy of selection programs. In addition, several resource populations are currently under development for the identification of QTL affecting carcass merit and pork quality. Initial results from one of these populations have revealed numerous QTL regions that can now be targeted for identification of the specific genes controlling the production of desirable pork products. The identification of specific genes having a major effect on growth, carcass merit, reproductive efficiency and health promises to facilitate the production of a significantly improved pork product at reduced costs. American agriculture will thus be more competitive on the world market.

Plans for the coming year:

Work planned by the Swine Genome Committee for next year includes further refinement of the pig comparative map. In addition, new anonymous markers will be identified and located on the genetic linkage map. Several stations are planning to sequence and map a significant number of ESTs. New resource populations are under development and will be used for QTL identification. Fine mapping of regions containing previously identified QTL will continue. Mapping and EST databases will be further refined.

Publications

Illinois:

Iowa:

  1. Ciobanu, D. C., A. E. Day, A. Nagy, R. Wales, M. F. Rothschild, and G. S. Plastow. 2000. Genetic variation of two local Romanian pig breeds assessed using DNA markers. NSIF Proceedings.
  2. Ciobanu, D., R. K. Gill, M. F. Rothschild, and N. H. Bell. 2000. Rapid Communication: Porcine vitamin D-25-hydroxylase maps to chromosome 5. J. Anim. Sci. 78:3193-3194.
  3. Ciobanu D. C, Gill R. K, Rothschild M. F, and Bell NH. 2000. Chromosomal localization of the porcine vitamin D-25-hydroxylase (CYP25) gene. In: Norman AW, Bouillon R, Thomasset M, eds. Vitamin D Endocrine System: Structural, Biological, Genetic and
  4. Didion, B. A., A. A. Paszek, G. A. Rohrer, H. S. Sun, and C. K. Tuggle. 2000. Rapid Communication: Genetic linkage and physical mapping of a porcine sequence characterized amplified region (SCAR) to chromosome 6. J. Anim. Sci. 78:3195-3196.
  5. Emnett, R. S., E. Grindflek, M. F. Rothschild, S. J. Moeller, D. L. Meeker, K. M. Irvin, and R. N. Goodwin. 2000. Peroxisomeproliferator-activated receptor( (PPAR() as a candidate gene for meat quality in swine. ASAS Midwest Meeting.
  6. Emnett, R., E. Grindflek, M. Rothschild, S. Moeller, D. Meeker, and K. Irvin. 2000. The effects of porcine peroxisome proliferator activated receptor ( (PPAR() in Berkshire, Duroc, Hampshire and Landrace breeds. PAG VIII proceedings P396.
  7. Emnett, R., S. Moeller, K. Irvin, M. F. Rothschild, G. Plastow and R. Goodwin. 2000. An Investigation into the Genetic Controls of Pork Quality. NSIF proceedings
  8. Emnett, R. S., S. J. Moeller, M. F. Rothschild, E. Grindflek, K. M. Irving, and D. L. Meeker. 2000. Physical assignment of adipocyte determination and differentiation factor-1 (ADD1) and pyruvate dehydrogenase E1-alpha (PDHA1) in the pig. Proceedin
  9. Fitzsimmons, C. J., L. Marklund, T. J. Stabel and C. K. Tuggle. 2000. Development of a real-time PCR detection method for the quantitation of MPO transcripts in porcine tissues. ISU Swine Research Reports.
  10. Gellin, J., S. Brown, J. Graves, M. Rothschild, L. Schook, J. Womack, and M. Yerle. 2000. Comparative gene mapping workshop: Progress in agriculturally important animals. Mammal. Genom. 11:140-144.
  11. Grapes, L., M. Malek, and M. F. Rothschild. 2000. Identification of identical twins and mutation rate in pigs. ISU Swine Research Reports.
  12. Grindflek, E., H. Sundvold, S. Lien, and M. F. Rothschild. 2000. Rapid Communication: Physical and genetic mapping of the peroxisome proliferator activated receptor (PPARG) gene to porcine chromosome 13: J. Anim. Sci. 78:1391-1392.
  13. Grindflek, E., J. Szyda, M. F. Rothschild, and S. Lien. 2000. A QTL analysis and candidate gene study for meat quality on swine chromosome 6. PAG VIII proceedings P399.
  14. Huff-Lonergan, E., T. J. Baas, M. Malek, J. Dekkers, K. Prusa, and M. F. Rothschild. 2000. Correlations among selected pork quality traits in a Berkshire by Yorkshire F2 population. J. Anim. Sci. 78 (Suppl. 1):160.
  15. Isler, B. J., K. M. Irvin, M. F. Rothschild, and G. J. Evans. 2000. Association between the prolactin receptor gene and reproductive components in swine. Proceedings of the 27th International Conference on Animal Genetics (Pg. 67).
  16. Kim, K. S., E. A. Mendez, S. Marklund, A. C. Clutter, D. Pomp, and M. F. Rothschild. 2000. Rapid Communication: Linkage mapping of the porcine Agouti gene. J. Anim. Sci. 78:1395-1396.
  17. Kim, K. S., M. Malek, D. Ciobanu, G. S. Plastow, and M. F. Rothschild. 2000. The association of an agouti-related protein (AGRP) gene polymorphism with growth and meat quality traits in commercial lines of pigs. Proceedings of the 27th Internationa
  18. Kim, K. S., N. J. Larsen, and M. F. Rothschild. 2000. Rapid Communication: Linkage and physical mapping of the porcine melanocortin-4 receptor (MC4R) gene. J. Anim. Sci. 78:791-792.
  19. Kim, K. S., N. Larsen, T. Short, G. Plastow, and M. F. Rothschild. 2000. A missense variant of the melanocortin 4 receptor (MC4R) gene is associated with fatness, growth and feed intake traits. Mammal. Genom. 11:131-135.
  20. Kim, K. S., S. Marklund, and M. F. Rothschild. 2000. Mapping of the porcine agarti related protein (AGRP) gene - a candidate gene for appetite and fatness. PAG VIII proceedings P405.
  21. Kim, K. S., S. Marklund, and M. F. Rothschild. 2000. The porcine melanocortin 5 receptor (MC5R) gene: polymorphisms, linkage and physical mapping. Anim. Genet. 31:228-441.
  22. Kim, K.-S., J. Sherwood, D. Ciobanu, Y. Zhang, and M. F. Rothschild. 2000. Mapping and investigation of two novel candidate genes for growth and meat quality traits in the pig. ISU Swine Research Reports.
  23. Lee, H. K., J. C. M. Dekkers, M. Malek, M. Soller, R. L. Fernando, and M. F. Rothschild. 2000. Comparison of approaches for determining significance threshold values for QTL detection. J. Anim. Sci. 78 (Suppl. 1):78.
  24. Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Baas, K. P. Prusa, E. Huff-Lonergan, and M. F. Rothschild. 2000. Identification of quantitative trait loci affecting meat quality in a Berkshire by Yorkshire 3 generation family. J. Anim. Sci. 78 (Suppl
  25. Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Baas, K. Prusa, E. Huff-Lonergan, and M. F. Rothschild. 2000. A molecular genome scan analysis to identify chromosomal regions influencing meat quality in the pig. Proceedings of the 27th International
  26. Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Baas, K. Prusa, E. Huff-Lonergan, and M. F. Rothschild. 2000. A molecular genome scan analysis to identify chromosomal regions influencing meat quality in the pig. Proceedings of 51st Annual Meeting of
  27. Malek, M., S. Marklund, C. Dyer, R. Matteri, and M. F. Rothschild. 2000. Linkage mapping of the porcine prepro-orexin gene. Mammal. Genom. 11:342-343.
  28. Malek, M., S. Marklund, C. Dyer, R. Matteri, and M. F. Rothschild. 2000. Linkage and physical mapping of the porcine prepro-orexin gene. PAG VIII proceedings P403.
  29. Malek, M, M. F. Rothschild, J.C.M. Dekkers, H.K. Lee, E. Huff-Lonergan, T.J. Baas, and K. Prusa. 2000. Quantitative trait loci analysis for growth and meat quality traits in the pig. Swine Research Reports
  30. Marklund, L., S. Kaul, and C. K. Tuggle. 2000. Rapid Communication: Mapping of the Myeloperoxidase (MPO) gene to pig chromosome 12. J. Anim. Sci. 78:3189-3190.
  31. Marklund, L., X. Shi, and C. K. Tuggle. 2000. Rapid Communication: Mapping of the Mannose-binding lectin 2(MBL2) gene to pig chromosome 14. J. Anim. Sci. 78:2992-2993.
  32. Marklund, S., C. K. Tuggle, and M. F. Rothschild. 2000. Mapping of CYPA1, SSTR1 and TTF1 genes to porcine chromosome 7q refines the porcine-human comparative map. Anim. Genet. 31:318-321.
  33. Navarro, P., R. K. Christenson, G. Ekhardt, B. Bosworth, J. K. Lunney, M. Rothschild, J. Lemke, and J. E. Butler. 2000. Genetic differences in the frequency of the hinge variants of porcine IgA is breed dependent. Vet. Immunol. Immunopath. 73:287-2
  34. Perez-Enciso, M., L. Varona, and M. F. Rothschild. 2000. Computation of identity by descent probabilities conditional on DNA markers via a Monte Carlo Marker Chain method. Genet. Sel. Evol. 32:467-482.
  35. Rothschild, M. F. 2000. Advances in pig molecular genetics, gene mapping, and genomics. X Reunion Nacional de Mejora Genetica Animal, Caldes de Montbui, June 8-9.
  36. Rothschild, M. F. 2000. Breakthrough genetic tests open new opportunities for Hampshire breeders. Seedstock Edge (July).
  37. Rothschild, M. F., L. A. Messer, A. Day, R. Wahs, T. Short, O. Southwood, and G. Plastow. 2000. Investigation of the retinol binding protein (RBP4) gene as a candidate gene for litter size in the pig. Mammal. Genom. 11:75-77.
  38. Rothschild, M., M. Malek, H. Lee, and J. Dekkers. 2000. A genome scan to identify QTL influencing obesity-related traits in the pig. Obesity Research 8 (Suppl. 1):60S
  39. Shi, X. W., C. J. Fitzsimmons, R. Prather, K. Whitworth, J. A. Green, and C. K. Tuggle. 2000. High-resolution physical mapping confirms similar gene order with an inversion between human 17 (Hsap17) and porcine chromosome 12 (Sscr12). ISU Swine Res
  40. Tuggle, C. K., S. Malchenko, R. Woods, K. Whitworth, J. A. Green, R. Prather, C. A. Roberts, C. J. Fitzsimmons, T. Casavant, and M. B. Soares. 2000. Development of new placental and fetal ESTs for gene discovery in pig production. ISU Swine Researc
  41. Tuggle, C. K., K. Whitworth, J. A. Green, R. Prather, M. F. Rothschild, D. Pomp, L. A. Messer, C. Roberts, T. Cassavant, and M. B. Soares. 2000. Gene discovery in porcine reproduction: physical mapping of 19 genes and ESTs. PAG VIII proceedings P5
  42. Walling, G. A., P. M. Visscher, L. Andersson, M. F. Rothschild, L. Wang, G. Moser, M. A. M. Groenen, J.-P. Bidanel, S. Cepica, A. L. Archibald, H. Geldermann, D. J. de Koning, D. Milan, and C. S. Haley. 2000. Combined analyses of data from QTL mappi
  1. Ciobanu, D. C., A. Nagy, R. Wales, A. E. Day, M. F. Rothschild, and G. S. Plastow. 2001. Genetic variation in two conserved local Romanian pig breeds using type 1 DNA markers. GSE (accepted).
  2. Grapes, L., M. Malek, and M. F. Rothschild. 2001. Identification of monozygous twins and microsatellite mutation rate in pigs from QTL linkage analysis data. J. Anim. Breed. Genet. (accepted).
  3. Grapes, L., Y. Zhang and M. F. Rothschild. 2001. Physical and linkage mapping of the porcine Connexin 37 gene. Proc. Plant and Animal Genome IX (in press)
  4. Grindflek, E., G. Plastow, and M. F. Rothschild. 2001. Mapping and investigation of the porcine major insulin sensitive glucose transport (GLUT4/SLC2A4) gene as a candidate gene for meat quality and carcass traits. Anim. Genet. (submitted).
  5. Grindflek, E. MF Rothschild, G Plastow, J Szyda and S Lien. 2001. Investigation of a Peroxisome Proliferator Activated Receptor ( (PPAR() polymorphism with candidate traits in pigs. Animal Genetics (submitted)
  6. Grindflek, E., J. Szyda, Z. Liu, M. F. Rothschild and S. Lien. 2001. Fine mapping of QTL affecting intramuscular fat on porcine chromosome 6: exclusion of H-FABP and MC5R as the causal genes for the QTL, J. Anim. Sci. (submitted)
  7. Kim, K. S., D. Ciobanu, G. Plastow, and M. F. Rothschild. 2001. Mapping of the porcine agouti-related protein (AGRP) gene and preliminary investigation of its genotypic effects on growth and meat quality traits. Anim. Genet. (submitted).
  8. Kim, K. S., J. Reecy, L. L. Anderson, and M. F. Rothschild. 2001. Functional characterization of the missense variation in the porcine Melanocortin-4 receptor gene associated with obesity-related traits in the pig. Proc. Plant and Animal Genome IX (i
  9. Lee, H., J. C. M. Dekkers, M. Malek, R. L. Fernando, and M. F. Rothschild. 2001. Comparison of approaches for determining significance threshold values for QTL mapping. Mammal. Gen. (submitted).
  10. Linville, R. C., D. Pomp, R. K. Johnson, and M.F. Rothschild. 2001. Candidate gene analysis for loci affecting litter size and ovulation rate in swine. J. Ani. Sci. (in press)
  11. Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Baas, and M. F. Rothschild. 2001. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. I. Growth and body composition. Mammal. Gen. (submitted).
  12. Malek, M., J. C. M. Dekkers, H. K. Lee, T. J. Baas, K. Prusa, E. Huff-Lonergan, and M. F. Rothschild. 2001. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition.
  13. Navarro, P., R. K. Christensen, P. Weber, M. Rothschild, G. Ekhardt, and J. E. Butler. 2001. Porcine IgA allotypes are not equally transcribed or expressed in heterozygous swine. Molec. Immunol. (submitted).
  14. Shi, X.-W., and Tuggle, C. K. 2000. Rapid Communication: Genetic linkage and physical mapping of the Porcine Phospholipid Transfer (PLTP) gene. J. Anim. Sci. (in press).
  15. Shi, X.-W., and Tuggle, C. K. 2000. Rapid Communication: Genetic and physical mapping of the porcine lipopolysaccaride binding protein (LBP) gene. J. Anim. Sci. (in press).
  16. Sherwood, J., K. S. Kim, D. Ciobanu, and M. F. Rothschild. 2001. Mapping of the porcine tropomyosin-beta gene a candidate gene for meat quality. Proc. Plant and Animal Genome IX (in press)
  17. Sponenberg, D. P. and M. F. Rothschild. 2001. Genetics of Colour and Hair Texture. In: Genetics of the Dog. Ed. A. Ruvinsky. CABI Press (in press).
  18. Tuggle, C. K., Genet, C., Shi, X.-W., Chardon, P., Sanchez-Serrano, I. L., Cravens, G., Milan, D., and Yerle, M. 2000. Cytogenetic and radiation hybrid mapping of human chromosome 21 genes in the pig. Mammalian Genome (in press).
  19. Yu, T.-P., H. S. Sun, S. Wahls, I. Sanchez-Serrano, M. F. Rothschild, and C. K. Tuggle. 2000. Cloning of the full length pit PIT1 (POU1F1) cDNA and a novel alternative PIT1 transcript, and functional studies of their encoded proteins. Anim. Biotech

Kansas:

  1. Wang Z, Rohrer G, Stone R, Troyer D: Isolation of thirty-one new porcine microsatellites from a microsatellite enriched microdissected chromosome 8 library. Animal Biotechnology 11(1):33-43, 2000.
  2. Wang Z, Rohrer GA, Stone RT, Troyer D: Twenty-six new porcine microsatellites from a microsatellite enriched microdissected chromosome 8 library. Journal of Animal Science, 78(12):3191-3192, 2000.
  3. Wang Z, Rohrer G, Stone R, Troyer D: Five new porcine genetic markers from a microsatellite enriched microdissected chromosome 13 library. Animal Genetics, In Press.

Michigan:

  1. Farber, C.R., N.E. Raney, K. Nadarajah, D.L. Kuhlers and C.W. Ernst. 2000. Identification of polymorphic loci between two pig populations using representational difference analysis. J. Anim. Sci. 78(Suppl. 2):27.
  2. Farber, C.R., N.E. Raney, K. Nadarajah, D.L. Kuhlers and C.W. Ernst. 2000. Identification and mapping of polymorphic loci between two pig populations using representational difference analysis. 27th International Conference on Animal Genetics. Minneap

Minnesota:

  1. Zhang, X., C. Wang, R.J. Hawken, L.B. Schook, L.J. Alexander, and M.S. Rutherford, 2000. A viral induced ubiquitin-specific protease (Ubp) localized on porcine chromosome 5. Mamm. Genome 11:340-341.
  2. Zhang, X., A. Wang, L.B. Schook, R. Hawken, and M.S. Rutherford, 2000. An RNA helicase gene, RHIV-1, induced by PRRS virus mapped on porcine chromosome 10q13. Microb. Pathogen. 28:267-278.
  3. Wang, C., R.J. Hawken, E. Larson, X. Zhang, L. Alexander, and M.S. Rutherford, 2000. Generation and mapping of expressed sequence tags from virus-infected swine macrophages. Anim. Biotechnol., in press.
  4. Gellin J., S. Brown, J.A. Marshall Graves, M. Rothschild, L. Schook, J. Womack, and M. Yerle, 2000. Comparative gene mapping workshop: progress in agriculturally important animals. Mamm. Genome 11:140-144.
  5. Barris, W. and R.J. Hawken, 2000. GelScore and Genetic Map Marker: two computer applications essential for radiation hybrid mapping. International Society of Animal Genetics Conference, Minneapolis, MN, July 22-27.
  6. Braunschweig, M.H., A.A. Paszek, R.J. Hawken, J.I. Weller, C.W. Beattie, L.B. Schook, and L.J. Alexander, 2000. Generation of a dense genetic map in a region of a QTL affecting corpora lutea in a Meishan x Yorkshire cross. International Society of An
  7. Hawken, R.J., G.H. Flickinger, S. Mikawa, N. Sarker, H. Yasue, L.J. Alexander, C.W. Beattie, and L.B. Schook, 2000. High resolution radiation hybrid map and YAC contig to define a QTL for average daily gain in swine. International Society of Animal Ge
  8. Erdman, C.A., M.J. Rebeiz, R.J. Hawken, L.B. Schook, H.A. Lewin, and J.E. Beever, 2000. A high-rsolution comparative gene map of pig chromosome 14. International Society of Animal Genetics Conference, Minneapolis, MN, July 22-27.
  9. Sarker, N., R.J. Hawken, S. Takahashi, S. Kiuchi, S. Mikawa, T. Awata, L.J. Alexander, L.B. Schook, and H. Yasue, 2000. Microdissection of chromosome one and high resolution gene mapping in the pig. International Society of Animal Genetics Conference
  10. Wang, C., R.J. Hawken, and M.S. Rutherford, 2000. Identification and assignment of PRRSV regulated genes in swine macrophages. Amer. Soc. Virol. 19th Annual Meeting, Fort Collins, CO, July 8-12.
  11. Wang, C., R.J. Hawken, E. Larson, X. Zhang, L. Alexander, and M.S. Rutherford, 2000. Generation and mapping of expressed sequence tags from virally infected swine cells. International Society of Animal Genetics Conference, Minneapolis, MN, July 22-2

Nebraska:

  1. Allan MF, D Pomp, MK Nielsen (2000) Gene Expression in Hypothalamus and Brown Adipose Tissue in Mice Selected for Heat Loss. Physiological Genomics 3:149-56.
  2. Cargill EJ, Happold TR, Lou MF, Pomp D, Nielsen MK (2000) Localization of a recessive juvenile cataract mutation to proximal chromosome 7 in mice. Human Heredity (In Press)).
  3. Cassady JP, RK Johnson, D Pomp, GA Rohrer, LD Van Vleck, EK Spiegel, KM Gilson (2001) Identification of Quantitative Trait Loci Affecting Reproduction in Pigs. J Anim Sci (In Press).
  4. Edeal B, Rumph JM, Mass R, Killinger K, Jerez N, Elnagar S, McDaneld T, Aljumaah R, Pithpongsiriporn U, Nephawe K, Martinez G, Gladney CD, Allan MF, Pomp D (2000) Linkage mapping of the Mahogany (attractin) locus in cattle and pigs. J Anim Sci 78:2479
  5. Kim KS, Mendez EA, Marklund S, Clutter AC, Pomp D, Rothschild MF (2000) Linkage mapping of the porcine Agouti gene. J Anim Sci 78:1395-6.
  6. Leamy L, Cheverud J, Eisen EJ, Pomp D (2000) Quantitative trait loci for directional but not fluctuating asymmetry of mandible characters in mice. Theor Appl Genet 76-27-40.
  7. Linville RC, D Pomp, RK Johnson, MF Rothschild (2000) Candidate Gene Analysis for Loci Affecting Litter Size and Ovulation Rate in Swine. J Anim Sci 79:60-67.
  8. Rocha J, Pomp D, Van Vleck D (2001) QTL Analysis in Livestock. In: Quantitative Trait Loci: Methods and Protocols. Methods in Molecular Biology. Eds; N Camp, A Cox (In Press).
  9. Pomp D (2000) Emerging technologies for genetic improvement of beef. Biotechnology: DNA. Beef Improvement Federation (Wichita).
  10. Pomp D (2000) DNA technologies for genetic improvement of beef. IRM Conference (Scottsbluff)
  11. Bertani GR, C Galdney, RK Johnson, D. Pomp (2000) Differentially expressed genes in anterior pituitary of pigs selected for reproduction. Plant and Animal Genome VIII, San Diego.
  12. Bertani GR, Gladney CD, Johnson RK, Pomp D (2000) Pig anterior pituitary EST's isolated by differential display in gene expression study of two lines selected for fertility. ISAG2000.
  13. Childs KD, Allan MF, Pomp D, Malayer JR, Geisert RD, Goad DW, MorganB (2000) Analysis of Adipocyte Gene Expression During Marbling in Angus X Hereford Steers. J Anim Sci.
  14. Edeal JB, Gladney CD, Allan MF, Pomp D, Jones SJ (2000) Identifying differentially expressed genes in C2C12 myogenic cells. J Anim Sci.
  15. Gladney C, GR Bertani, RK Johnson, D. Pomp (2000) Evaluation of gene expression in ovarian follicles of pigs selected for reproduction using microarray and differential display PCR technologies. Plant and Animal Genome VIII, San Diego.
  16. Gladney CD, Bertani GR, Johnson RK, Pomp D (2000) Differential display PCR and microarray evaluation of ovarian follicle gene expression in pigs selected for reproduction. ISAG2000.
  17. Rocha JL, Pomp D, Van Vleck LD (2000) On criteria of marker-informativeness in an F2/outbred cross context. ISAG2000.
  18. Siewerdt F, Eisen EJ, Pomp D (2000) Genome scan for quantitative trait loci for growth and reproductive traits in female mice. J Anim Sci.

USDA-BARC:

  1. Navarro, P., Christenson, R., Ekhardt, G., Bosworth, B., Lunney, J. K., Rothschild, M., Lemke, J., and Butler, J. E. 2000. Genetic differences in the frequency of the hinge variants of porcine IgA is breed dependent. Vet. Immunol. Immunopathol. 73: 2
  2. Alvarez, B., Sanchez, C., Bullido, R., Marina, A., Lunney, J., Alonso, F., Ezquerra, A., and Dominguez, J. 2000. Porcine SWC3 is a member of the SIRP family and associates with protein-tyrosine phosphatase SHP-1. Tissue Antigens. 55: 342-351.
  3. Solano-Aguilar G.I., Vengroski K, Beshah E., and Lunney J.K. Isolation and Purification of lymphocyte subsets from gut associated lymphoid tissue in neonatal swine. J. Immunol. Methods. 241: 185-199.
  4. Solano-Aguilar G.I., Beshah E., Vengroski K., Zarlenga D., Jauregui L., Cosio M., Douglass L.W., Dubey J.P., and Lunney J.K. 2000. Cytokine and Lymphocyte profile after Toxoplasma gondii infection in miniature swine. Proc. 16th Cong. Intnl. Pig Vet. S
  5. Solano-Aguilar G.I., Urban J.F., Beshah E., Schopf L., Kringel H., Domingo A., Dubey J.P., and Lunney J.K. 2000. Mucosal immune response of young pigs after Toxoplasma gondii infection. Proc. 16th Cong. Intnl. Pig Vet. Soc., Australia. P.171.
  6. Solano-Aguilar G.I., Beshah E., Vengroski K., Zarlenga D., Jauregui L., Cosio M., Douglass L.W., Dubey J.P. and Lunney J.K. 2001. Cytokine and lymphocyte profile in miniature swine after oral infection with Toxoplasma gondii oocysts. Intnl. J. Parasit
  7. Johnson P., Keen N.T., Lunney J.K., and Sadowsky M.J. 2001. Meeting Editorial: Agricultural Microbes Genome 2. Comparative and Functional Genomics. In Press
  8. Solano-Aguilar, G.I., Vengroski, K, Beshah, E., Douglass, L.W., and Lunney, J.K. 2000. Characterization of lymphoid populations from mucosal tissues of neonatal pigs. Dev. Comp. Immunol. 25: 245-263.

USDA-MARC:

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