Obtaining Primer Sequences To Amplify The Gene

• Most challenging aspect of candidate gene analysis.

• Can obtain some sequence information from the species of interest or related species in existing databases.

• Use Genbank or EMBL for sequence information.

  • http://www.ncbi.nlm.nih.gov/Genbank/GenbankSearch.html
  • http://srs.ebi.ac.uk/srs5bin/cgi-bin/wgetz?-page+LibInfo+-id+4Flds1Ecs1e+-lib+EMBL

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