Identification of important genes Candidate gene analysis
Introduction
Introduction (Cont.)
Candidate Genes -Definitions-
PPT Slide
Other Candidate Genes -Definitions continued-
The Hypothesis
Candidate Gene Approach Methodologies
Candidate Gene Approach Methodologies (cont.)
Choosing The Candidate Gene
Candidate Genes -Sources for Genes-
Choosing The Candidate Gene (Cont.)
Candidate Genes -Sources for Positional CandidateGenes-
Obtaining Primer Sequences To Amplify The Gene
Obtaining Primer Sequences To Amplify The Gene (Cont.)
Uncovering Polymorphisms In the Candidate Genes
Uncovering Polymorphisms in the Candidate Genes (Cont.)
Developing Large Scale Genotyping
Identifying A Population For Association Studies
Carrying Out An Association Study of Candidate Gene With Trait Phenotype
Carrying Out An Association Study of Candidate Gene With Trait Phenotype (Cont.)
Verifying Associations That Are Uncovered
Verifying Associations That Are Uncovered (Cont.)
Examples of Candidate Genes
Meishan Sow from China
Why so many pigs?
ESR - a good candidate gene?
ESR PCR-RFLP Test
Effect of ESR on Litter Size Meishan Synthetics
ESR Means for Reproduction Traits ~5,000 LW females
ESR Means for Production Traits
What Does ESR Do?
Prolactin (PRL)
PRLR
Latest PRLR Allelic Effects
Candidate Gene Hunt: Embryo development
Candidate Gene Hunt: Why RBP4?
RBP4 Overall Total Number Born
Knockout Mutations
Double muscling
Myostatin-mouse model
Putative System for Regulation of Energy Balance
Gene Identification for Traits: MC4R
MC4R mutation and Test
Sequence information
Examples - Conclusions
Final Thoughts
Advantages and Limitations of the Candidate Gene Approach
Advantages (Cont.)
Limitations (Cont.)
Cautionary Thoughts
Cautionary Thoughts (Cont.)
Conclusions and Implications
Email: mfrothsc@iastate.edu
Home Page: /edu/QTL/rothschild/