From listmaster animalgenome.org Wed Jan 15 11:29:39 2020
From: Laura Iacolina <lauraiacolinagmail.com>
Subject: Re: DNA extraction from cattle hair for WGS
To: Members of AnGenMap <angenmapanimalgenome.org>
Date: Wed, 15 Jan 2020 11:29:39 -0600
Liz, is it stable? We sometimes have problem with Qiagen kits (never tried
the Gentra though) and sources as hair and bone. Results are ok, but we
need to perform the analysis within a short time 7-10 days otherwise it
degrades and is no longer suitable.
Thanks,
Laura
--
PhD Laura Iacolina
Mammalian Biology subject editor
https://www.researchgate.net/.../Laura_Iacolina/info
https://lauraiacolina.wordpress.com/
Il giorno mer 15 gen 2020 alle ore 18:16 Elizabeth Ross <mailto:e.rossuq.edu.au> ha scritto:
We use Gentra Puregene Tissue Kit from qiagen on tail hair and use the DNA
for nanopore sequencing. It gives great purity and excellent length if you
are gentle with it.
Cheers
Liz
On Jan 16, 2020 1:32 AM, Rick Tearle <mailto:rick.tearleadelaide.edu.au> wrote:
Does anyone have a good method for extracting DNA from cattle hair?
We need it for whole genome sequencing so has to be high MW.
Methods that work for genotyping do not appear to work for WGS,
Dr Rick Tearle
Senior Bioinformatician
Davies Research Centre
University of Adelaide Roseworthy Campus
rick.tearleadelaide.edu.au
+61 432 07 58 07
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