From paolo.zambonelli unibo.it Thu Apr 14 07:25:04 2016
From: Paolo Zambonelli <paolo.zambonelliunibo.it>
To: Multiple Recipients of AnGenMap <angenmapanimalgenome.org>
Subject: R: RNA extraction from adipose tissues
Date: Thu, 14 Apr 2016 07:25:04 -0500
Dear Juha,
in my experience the classical guanidine-thyocianate metod is the best to
extract RNA from porcine adipose tissue. The quality of the RNA extracted is
suitable for RNA-seq but is not excellent. We performed RNA-seq experiments
to analyze both mRNAs and miRNAs.
Best regards,
Paolo Zambonelli
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Dr. Paolo Zambonelli, PhD
Universita' di Bologna - DISTAL
Via Fratelli Rosselli, 107
I-42123 Reggio Emilia - Italy
tel +39 051 20 88055
paolo.zambonelliunibo.it
skype paolo.zzz
http:// www.unibo.it/docenti/paolo.zambonelli
======================================
-----Messaggio originale-----
Da: Juha Kantanen [mailto:juha.kantanenluke.fi]
Inviato: mercoled 13 aprile 2016 15:16
A: Multiple Recipients of AnGenMap
Oggetto: RNA extraction from adipose tissues
Dear AnGenMap members,
Your experiences in RNA extraction from adipose tissues of various domestic
animal species are needed, which RNA extraction protocol works best. Some
commercial kit? Or perhaps you have developed your own succesful protocol.
Are there differences between species and some method works well in cattle
and less optimally e.g. in horse or vice versa? Our aim is to sequence both
mRNA and miRNA. Thank you for your comments and help!
With best regards Juha Kantanen ********** Juha Kantanen, PhD, Professor
Natural Reseources Institute Finland Myllytie 1 FI-31600 Jokioinen, Finland
GSM +358 29 5326210
Please note the current address: Department of Biology University of Eastern
Finland PO Box 1627 FI-70211 Kuopio, Finland
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