From jennifer.thomson montana.edu Wed Apr 13 20:58:59 2016
From: "Thomson, Jennifer" <jennifer.thomsonmontana.edu>
Subject: Re: RNA extraction from adipose tissues
To: Multiple Recipients of AnGenMap <angenmapanimalgenome.org>
Date: Wed, 13 Apr 2016 20:59:00 -0500
Dear Juha,
I have had the best results using the Rneasy Lipid Tissue Mini Kit from
Qiagen when extracting RNA from adipose and brain tissue in cattle and sheep.
The quality and quantity was sufficient for RNA-seq.
Regards,
Jennifer Thomson
Jennifer Thomson
Asst. Professor -- Animal Genetics/Genomics
Department of Animal and Range Sciences
Montana State University
Bozeman, MT 59717
(406) 994-7434
On 4/13/16, 7:16 AM, "Juha Kantanen" <juha.kantanenluke.fi> wrote:
> Dear AnGenMap members,
>
> Your experiences in RNA extraction from adipose tissues of various
> domestic animal species are >needed, which RNA extraction protocol
> works best. Some commercial kit? Or perhaps you have developed your
> own succesful protocol. Are there differences between species and
> some method works well in cattle and less optimally e.g. in horse
> or vice versa? Our aim is to sequence both mRNA and miRNA. Thank
> you for your comments and help!
>
> With best regards
> Juha Kantanen
> **********
> Juha Kantanen, PhD, Professor
> Natural Reseources Institute Finland
> Myllytie 1
> FI-31600 Jokioinen, Finland
> GSM +358 29 5326210
>
> Please note the current address:
> Department of Biology
> University of Eastern Finland
> PO Box 1627
> FI-70211 Kuopio, Finland
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