From capemaster gmail.com Mon Jun 22 12:34:41 2015
From: Stefano Capomaccio <capemastergmail.com>
Subject: Re: RNA extraction from adipose tissues
To: Multiple Recipients of AnGenMap <angenmapanimalgenome.org>
Date: Mon, 22 Jun 2015 12:34:41 -0500
Dear Juha,
from my experience one commercial kit that works well is the Aurum Fatty
and Fibrous from Bio-Rad.
In alternative, you can grind with liquind nitrogen and and follow a
classic TriZol extraction methos.
It really depends by the amount of tissue you have and how many samples:
classic way is labour intensive.
Hope this helps,
Stefano
On Mon, Jun 22, 2015 at 2:43 PM, Juha Kantanen <juha.kantanenluke.fi>
wrote:
> Dear all AnGenMap members,
>
> Do you have suggestions for methods of RNA extraction (commercial kits,
> some longer method) from subcutaneous, scapular and perirenal adipose
> tissues? Thanks a lot for your help.
>
> With best regards Juha Kantanen
>
> **************
> Juha Kantanen, PhD, Professor
> Natural Resources Institute Finland
> Myllytie 1
> FI-31600 Jokioinen, Finland
> juha.kantanenluke.fi
>
> Please note the current address:
> Department of Biology
> University of Eastern Finland
> PO Box 1627
> FI-70211 Kuopio, Finland
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*He tried to be a scientist*
Stefano Capomaccio, PhD
Istituto di Zootecnica
Università Cattolica del Sacro Cuore
Via Emilia Parmense, 84
29122 - Piacenza (PC), Italy
Phone +39 0523 599203 (office)
Phone +39 0523 599482 (lab)
email: stefano.capomacciounicatt.it
email: capemastergmail.com
skype: capemaster
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