From landivincenzo yahoo.it Wed Sep 17 08:20:34 2014
From: Vincenzo Landi <landivincenzoyahoo.it>
To: Multiple Recipients of <angenmapanimalgenome.org>
Subject: DNA extraction from horse hair
Date: Wed, 17 Sep 2014 08:20:34 -0500
Dear Mohamed,
I my lab we extract DNA for parentage testing purpose whit the following
method:
1) 3 hair roots in a PCR tube
2) add 100ul of 10mM Tris, 0,01 Tween20 (you can substitute whit a
simple PCR Buffer)
3) add 5 ul of Protease K 20mg/ul
4) incubate at 60 degree for 1 hour and at 95 degree for 15 minutes
you can use directly
Best regards
*************************************************************
Vincenzo Landi, Phd
Laboratorio de Gen�tica Molecular Aplicada
Departamento de Gen�tica, Universidad de C�rdoba
Edificio Gregor Mendel, Planta Baja
Campus Universitario de Rabanales
14071-C�rdoba (Spain)
Tf: +34957218708, +34630639439
FAX: +34957218706
http://www.biolabo.es
Il 17/09/2014 6:33, banabazi ha scritto:
> we
> added DTT (Dithiothreitol) to hairs. Then, we had a gelatin-like
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