From kamauvet yahoo.com Tue Jan 31 02:27:35 2012
From: Joseph Kamau <kamauvetyahoo.com>
To: Multiple Recipients of <angenmapanimalgenome.org>
Subject: How to Extract Leishmania DNA from blood of an infected mouse?
Date: Tue, 31 Jan 2012 02:27:35 -0600
Dear all,
I am looking for a DNA extraction method that can be used to extract
Leishmania parasite DNA from blood of an infected mouse. I have tried using
Quick-gDNA MiniPrep, but the DNA concentration is too low to be detected by
even LAMP or Nested PCR.
When the kit is applied on liver and spleen tissues of the same infected
mouse, the amount of parasite DNA extracted is quite much, but tissue
biopsies cant be the routine diagnostic method of choice. Blood would be
the better option if we would find a way of extracting sufficient parasite
DNA.
Some people have recommended going for RNA as opposed to DNA, that parasite
RNA would be much! am yet to understand the rationale.....
kindly anyone out there who can help navigate the problem?
Sincerely,
Joseph Kamau, PhD
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