From m.shariflou usyd.edu.au Fri Dec 7 09:19:49 2007
Date: Fri, 7 Dec 2007 23:51:51 +1100
From: "Mohammad Shariflou" <m.shariflouusyd.edu.au>
To: Multiple Recipients of <angenmapanimalgenome.org>
Subject: RE: long term storage of cattle blood for DNA isolation
Dear James,
FTA cards can be stored in RT and you will only need very small volumes of
blood. If large quantities of DNA are needed, whole genome amplification is
a good option, although there is a small chance of losing some loci.
For storing large volumes, I suggest splitting samples into small volumes
(depending on how much you need for DNA extraction) and keeping them
separately for ease of handling and for securities.
If you need to keep them for up to 4 weeks, you can store them in -20C. We
have found that for samples frozen at -80C, high salt method works better
and is easier compared to ph/ch method and a commercial kit.
Regards
Mohammad
Mohammad Reza Shariflou, PhD
Senior Researcher, SheepGenomics
Reprogen, Faculty of Veterinary Science
Level 5, Room 513, Gunn, B19
The University of Sydney
NSW 2006, Australia
> From: James E Koltes [mailto:jekoltesiastate.edu]
> Sent: Thu 6/12/2007 8:02 AM
> To: Multiple Recipients of
> Subject: long term storage of cattle blood for DNA isolation
>
> I am looking for a way to preserve blood samples (10-20mL) from
> cattle for an extended period of time (1-4 weeks) prior to DNA
> isolation.
>
> We are currently using purple top, EDTA tubes for blood sample
> collection.
> Then, we isolate the buffy coat using red blood cell lysis buffer and
> PBS
> incubation/ washes for downstream DNA extraction. This is not an ideal
> situation, as isolating DNA sooner is not an option. Any suggestions for
> archival of blood or DNA extraction methods that would improve DNA yield
> on
> archived samples would be greatly appreciated.
>
> James
>
>
> James Koltes
> Interdepartmental Genetics
> Iowa State University
> 2361 Kildee Hall
> Ames, IA 50011-3150 USA
> Phone:515-294-9086
> jekoltesiastate.edu
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