From mnhiyer yahoo.co.in Mon Mar 19 01:11:39 2007
Date: Mon, 19 Mar 2007 06:11:37 +0000 (GMT)
From: mohan iyer <mnhiyeryahoo.co.in>
Subject: Detection of gene promoters
To: Multiple Recipients of <angenmapanimalgenome.org>
Dear Sir,
I wish to detect promoter for some genes related to
fatty acid synthesis. can any body help by suggesting
me some techniques for it. Reviews or references will
be of good help.
--- Joel Weller <welleragri.huji.ac.il> wrote:
> Dear Mohamad,
>
> Is there a SNP chip for sheep? I could not
> find one.
>
> Joel Weller
>
> At 02:27 15/03/2007, you wrote:
>
> > Dear AnGenMappers
> >
> > We are investigating a suitable protocol for
> > extraction of DNA from a large
> > number of sheep blood samples. The main goal is to
> > use these DNA samples
> > for SNP genotyping, using a SNP chip platform.
> >
> > Different service providers require DNA products
> > resulted from their own
> > recommended protocol or DNA extraction kit. This
> > means, extraction of only
> > sufficient amounts of DNA at a time for any
> > particular genotyping
> > application. As multiple extraction procedure is
> > not cost effective, we are
> > looking for an alternative method.
> >
> > Ideally we want to extract large quantities of DNA
> > in one go, with the view
> > of having sufficient DNA for our current SNP
> > genotyping needs and also for
> > storing for other applications/genotyping
> > platforms in the future. We need
> > to find an answer for the following question.
> >
> > Is there any generic protocol or DNA extraction
> > kit, so that the resulting
> > DNA can be used for genotyping platforms offered
> > by different service
> > providers?
> >
> > Any comments and suggestions on related protocols,
> > commercial kits, or
> > other options are most welcomed and highly
> > appreciated.
> >
> > Regards Mohammad
Dr.Mohan.N.H,
Scientist
National Research Centre on Pig,
Indian Council of Agricultural Research,
By lane No 2 (north), Panjabari Road,
Guwahati,
Assam 781 022. India
Ph:0361-2333359
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