From weller agri.huji.ac.il Thu Mar 15 16:55:49 2007
Delivered-To: anganmap animalgenome.org
Date: Thu, 15 Mar 2007 16:37:01 +0200
From: Joel Weller <weller agri.huji.ac.il>
Subject: Fwd: Re: DNA extraction protocols/kits for SNP genotyping
To: Multiple Recipients of <angenmap animalgenome.org>
Dear Mohamad,
Is there a SNP chip for sheep? I could not find one.
Joel Weller
At 02:27 15/03/2007, you wrote:
> Dear AnGenMappers
>
> We are investigating a suitable protocol for extraction of DNA from a large
> number of sheep blood samples. The main goal is to use these DNA samples
> for SNP genotyping, using a SNP chip platform.
>
> Different service providers require DNA products resulted from their own
> recommended protocol or DNA extraction kit. This means, extraction of only
> sufficient amounts of DNA at a time for any particular genotyping
> application. As multiple extraction procedure is not cost effective, we are
> looking for an alternative method.
>
> Ideally we want to extract large quantities of DNA in one go, with the view
> of having sufficient DNA for our current SNP genotyping needs and also for
> storing for other applications/genotyping platforms in the future. We need
> to find an answer for the following question.
>
> Is there any generic protocol or DNA extraction kit, so that the resulting
> DNA can be used for genotyping platforms offered by different service
> providers?
>
> Any comments and suggestions on related protocols, commercial kits, or
> other options are most welcomed and highly appreciated.
>
> Regards Mohammad
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Joel Ira Weller * * *
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