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To: angenmap <angenmapiastate.edu>
From: Steve Kemp <S.J.Kempliverpool.ac.uk>
Subject: Re: ANimal GENe MAPpers Discussion Group
Does anyone have a simple method for crude DNA preparations from
cryoprserved bovine semen ? I have tried chelex and NaOH lysis and both fail
to produce amplifiable DNA. If I follow these with phenol extraction and
precipitation it amplifies OK, but this is too slow for large numbers of
samples. There seems to be something in the semen or the cryopreservation
medium which inhibits taq polymerase. Has anyone any ideas ?
Thanks
Steve Kemp
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Stephen J Kemp
Department of Genetics & Microbiology
University of Liverpool
Liverpool L69 3BX
UK
Tel: 0151-794-3618/3622
Fax: 0151-794-3655
email: KEMPSJLIV.AC.UK
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